Job Description
Position Summary
The Lead Discovery and Structural Biology Department is seeking a highly qualified Protein Expression & Purification Scientist with demonstrated expertise in molecular biology, heterologous protein expression, and protein purification. The successful candidate will contribute to research programs within an industry-leading drug discovery environment leveraging access to state-of-the-art protein expression and purification platforms. Responsibilities include executing expression workflows in baculovirus expression vector systems (BEVS)/insect cells, as well as in E. coli, including high-throughput (HTP) small-scale expression screening, followed by downstream purification and analytical characterization. Experience with biophysical methods is considered an asset.
Essential Duties and Responsibilities
- Perform molecular biology workflows, including:
- Cloning, vector design, construct optimization, and sequence verification
- PCR, primer design, restriction based, and recombination-based cloning
- Execute protein expression using:
- BEVS/insect cell systems, including virus generation, amplification, and expression optimization
- E. coli expression, including IPTG induced and auto-induction strategies
- High throughput (HTP) small scale expression testing for rapid screening of constructs and conditions
- Conduct purification of recombinant proteins using standard chromatographic techniques (affinity, ion exchange, size exclusion chromatography).
- Optimize expression constructs, culture conditions, and purification workflows to achieve the required yield, purity, and quality.
- Perform analytical characterization, including SDS PAGE, Western blotting, SEC, and other relevant assays.
- Maintain accurate and complete laboratory records in accordance with internal documentation standards (experience with Benchling is a plus).
- Communicate experimental results, observations, and recommendations to team members in a clear and timely manner.
- Adhere to all laboratory safety policies and organizational compliance requirements.
Education and Experience
- Bachelor’s degree in Biochemistry, Molecular Biology, Structural Biology, or related field with 3+ years of relevant industry experience, or
- Master’s degree with 1+ year of relevant industry experience
- Strong molecular biology and cloning expertise is required.
- Proven track record of protein expression in BEVS/insect systems and E. coli, including HTP small scale expression screening.
- Demonstrated proficiency in chromatographic purification and method development.
- Experience with biophysical characterization techniques (e.g., LC?MS, SPR, MST, SEC?MALS, nanoDSF, mass photometry) is preferred but not required.
Knowledge, Skills, and Abilities
- Strong analytical and troubleshooting skills across cloning, expression, and purification workflows.
- Ability to work independently while contributing and collaborating effectively with cross-functional teams.
- Excellent organizational, documentation, and communication skills.
- Ability to manage multiple tasks and meet project timelines in a fast-paced environment.